Biophysics of Membrane Proteins
My primary research focuses on the biophysical mechanisms of membrane proteins, particularly channels and secondary active transporters. I especially like to use biochemical, biophysical, and structural techniques (CryoEM and X-ray) to describe the mechanism of each protein, their targeting by small molecules, and the effects of disease mutations.
Li et al. Structure and function of the SIT1 proline transporter in complex with the COVID-19 receptor ACE2 (submitted)
Sauer et al. Structural basis of ion – substrate coupling in the Na+-dependent dicarboxylate transporter VcINDY. Nature Communications (2022)
Brawley, Sauer, et al. Structural basis for inhibition of the drug efflux pump NorA from Staphylococcus aureus. Nature Chemical Biology (2022)
Sauer et al. Structure and inhibition mechanism of the human citrate transporter NaCT. Nature (2021)
Sauer et al. Structural basis for the reaction cycle of DASS dicarboxylate transporters. eLife (2020)
Sauer et al. Sodium and potassium competition in potassium-selective and non-selective channels. Nature Communications (2013)
Derebe, Sauer, et al. Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites. PNAS (2011)
I also have an interest in computational biology, especially as it relates to structural biology, thermoadaptation, and reverse ecology. Of particular interest is the application of computational tools to structural biology, or aiding the interpretation of structural and biochemical results.
Sauer and Wang, Using machine learning to predict quantitative phenotypes from protein and nucleic acid sequences
Sauer and Wang, Predicting the Optimal Growth Temperatures of Prokaryotes using only Genome Derived Features. Bioinformatics (2019)
Sauer et al. Rapid Bioinformatic Identification of Thermostabilizing Mutations. Biophysical Journal (2014)